High-Throughput Screening of Type III Secretion Determinants Reveals a Major Chaperone-Independent Pathway
نویسندگان
چکیده
منابع مشابه
Salmonella type III secretion-associated chaperones confer secretion-pathway specificity.
Type III protein secretion systems (TTSSs) are ancestrally related to the flagellar export system and are essential for the virulence of many bacteria pathogenic for humans, animals and plants. Most proteins destined to travel the TTSS pathway possess at least two domains that specifically target them to the secretion apparatus. One of the domains is located within the amino terminal first appr...
متن کاملStructure of AcrH-AopB Chaperone-Translocator Complex Reveals a Role for Membrane Hairpins in Type III Secretion System Translocon Assembly.
Type III secretion systems (T3SSs) are adopted by pathogenic bacteria for the transport of effector proteins into host cells through the translocon pore composed of major and minor translocator proteins. Both translocators require a dedicated chaperone for solubility. Despite tremendous efforts in the past, structural information regarding the chaperone-translocator complex and the topology of ...
متن کاملHypoxia response: a model toxicity pathway for high-throughput screening.
The identification of toxicity pathways associated with known adverse health effects combined with engineered cellular assays that measure perturbations of these pathways is the keystone to the successful implementation of the recently formulated National Research Council (2007) report, Toxicity Testing in the 21st Century: A Vision and a Strategy. While the toxicology community probes biologic...
متن کاملHigh-throughput RNAi screening reveals novel regulators of telomerase.
Telomerase is considered an attractive anticancer target on the basis of its common and specific activation in most human cancers. While direct telomerase inhibition is being explored as a therapeutic strategy, alternative strategies to target regulators of telomerase that could disrupt telomere maintenance and cancer cell proliferation are not yet available. Here, we report the findings of a h...
متن کاملTranscriptional profiling of Bordetella pertussis reveals requirement of RNA chaperone Hfq for Type III secretion system functionality
Bordetella pertussis, the causative agent of human whooping cough (pertussis) produces a complex array of virulence factors in order to establish efficient infection in the host. The RNA chaperone Hfq and small regulatory RNAs are key players in posttranscriptional regulation in bacteria and have been shown to play an essential role in virulence of a broad spectrum of bacterial pathogens. This ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: mBio
سال: 2018
ISSN: 2161-2129,2150-7511
DOI: 10.1128/mbio.01050-18